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Decoding the human genome resulted in generating large amount of data that need to be analyzed and given a biological meaning. The field of Life Schiences is highly information driven. The genomic dat...
The heart is the first organ to form during development in vertebrates and Nkx2-5 is the first marker of cardiac specification. In Xenopus laevis, Nkx2-5 is essential for heart formation, but early ta...
Decoding the human genome resulted in generating large amount of data that need to be analyzed and given a biological meaning. The field of Life Schiences is highly information driven. The genomic dat...
Copy number variations (CNVs) are gains and losses of DNA se-quence of a genome, which result in the abnormality of the cell or individual. The data from single nucleotide polymorphism (SNP) microar...
The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of pure...
When analyzing microarray data, hierarchical models are often used to share information across genes when estimating means and variances or identifying differential expression. Many methods utilize so...
The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significa...
In this work, we modify the superparamagnetic clustering algorithm (SPC) by adding an extra weight to the interaction formula that considers which genes are regulated by the same transcription factor....
Estimation of genewise variance arises from two important applications in microarray data analysis: selecting significantly differentially expressed genes and validation tests for normalization of mi...
In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized compleme...
We created a cDNA microarray representing approximately 3,500 pig genes for functional genomic studies. The array elements were selected from 6,494 cDNA clones identified in a large-scale expressed se...
摘要对寡核苷酸DNA Microarray用于HLADRB1基因分型的技术进行研究。常规的酚/氯仿法提取标准血样基因组DNA,在DRB1的exon2区域设计一对引物,经PCR扩增基因组相应区段并用Cy5-dCTP进行标记。设计寡核苷酸分型探针,将探针固定在APS-PDC法制作的DNA Microarray上,用标记的PCR产物与之杂交,扫描仪对杂交结果进行扫描,Imagene软件对杂交图象进行分析...
摘要为了优化筛检cDNA微阵列中靶基因的最适长度、浓度及点样溶液的种类,设计持家基因beta actin和GAPDH RT-PCR 3对引物,产物长度在189~1 078 bp之间,以乙肝病毒DNA片段为阴性对照,扩增纯化后分别溶于3×SSC、50%DMSO及0.5mol/L碳酸盐缓冲液(pH=9.0)中,调整浓度分别为0.5μg/μL、1.0μg/μL和1.5μg/μL,比较上述不同条件的杂交结...
专著信息 书名 laser capture microdissection combined functional grouping cDNA microarray analysis of the angiogenesis in cerebral ischemic 语种 英文 撰写或编译 作者 Tian YF,Liu Y,Zhang PB,Zhang JS,Qi CF 第一作者单位 出版社 Bra...
专著信息 书名 Fabrication of double-stranded DNA microarray on solid surface for studying DNA-protein interactions: a high-throughput platform for profiling bimolecular interaction. 语种 英文 撰写或编译 作者 Jinke Wan...

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