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Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Enhancement of the Fluorescence of the Blue Fluorescent Proteins by High Pressure or Low Temperature
Enhancement Fluorescence Blue Fluorescent Proteins High Pressure Low Temperature
2016/5/24
Green fluorescent proteins bearing the Y66H mutation exhibit strongly blue-shifted fluorescence excitation and emission spectra. However, these blue fluorescent proteins (BFPs) have lower quantum yiel...
Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy
Water Oligonucleotides Microscopy, Fluorescence Microscopy, Interference Membranes, Artificial Sensitivity and Specificity Surface Properties Models, Chemical Silicon Dioxide
2016/5/24
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Electric Field Modulation of the Fluorescence Spectrum from Rhodobacter Sphaeroides Reaction Centers
fluorescence counterstain nuclear counterstain immunocytochemistry propidium iodide cell culture
2016/5/20
The fluorescence intensity from randomly oriented Rhodobacter ( Rb.) sphaeroides photosynthetic reaction centers at 77 K increases in an applied electric field. This is ascribed to a net reduction in ...
Fluorescence Lifetimes Measurements Using a Synchrotron Source
Fluorescence Lifetimes Measurements Synchrotron Source
2016/5/20
Fluorescence Lifetimes Measurements Using a Synchrotron Source.