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Ethanol accelerates acrosomal loss in human spermatozoa
Ethanol accelerates acrosomal loss human spermatozoa
2009/2/23
The effects of ethanol on the loss of the human sperm acrosome, as determined by the chlortetracycline fluorescence assay and by indirect immunofluorescence assay, were assessed over 6 hours during ...
Immunocytochemical localization of acrosin on both acrosomal membranes and in the acrosomal matrix of porcine spermatozoa
spermatozoa acrosin electron microscopy
2009/2/19
Immunocytochemical techniques were employed to determine, at the ultrastructural level, the location of acrosin in porcine spermatozoa. Antisera to highly purified porcine acrosin was produced in ...
Deficiency of fucosidase results in acrosomal dysgenesis and impaired sperm maturation
Fucosidosis spermatogenesis epididymis dog
2009/2/17
Although a variety of glycosyltransferases and glycosidases have been implicated in spermatogenesis and posttesticular sperm maturation, the biological role of these enzymes in these processes is larg...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
Voltage-dependent calcium channels and Gi regulatory protein mediate the human sperm acrosomal exocytosis induced by N-acetylglucosaminyl/mannosyl neoglycoproteins
Carbohydrate G-protein acrosome reaction VDCC neoglycoproteins
2009/2/16
Mammalian spermatozoa must undergo an exocytotic event during fertilization, the acrosome reaction (AR). In most species studied this process is induced by specific glycoproteins of the oocyte ext...
Functional characterization of the primate sperm acrosomal antigen（PSA-63）
Functional characterization primate sperm acrosomal antigen
2009/2/13
Monoclonal antibody (HS-63) raised in mice against human ejaculated sperm, polyclonal antibodies raised in rabbits against the cognate mouse testicular antigen (MSA-63; or Fab) and polyclonal antibodi...
Phosphatidylcholine enhances the acrosomal responsiveness of human sperm
Phosphatidylcholine enhances acrosomal responsiveness human sperm
2009/2/12
Supplementing bovine serum albumin-containing medium with phosphatidylcholine (PC) accelerated the in vitro development of human sperm acrosomal responsiveness. Responsiveness was assessed by expo...
Nanovid microscopy for assessing sperm membrane changes induced by in vitro capacitating and acrosomal reacting procedures
Spermatozoa colloidal gold videomicroscopy
2009/2/12
This study was to verify the usefulness of Nanovid microscopy techniques for evaluating induced modifications in bovine spermatozoal membranes. Frozen thawed bovine sperm were labeled with 20-nm c...
Validation of an acrosomal stain for equine sperm that differentiates between living and dead sperm
acrosomal stain equine sperm living and dead sperm
2009/2/12
An acrosomal staining technique that can differentiate between living and dead sperm was developed for equine sperm. The fluoresceinated lectin Pisum sativum agglutinin (FITC-PSA) was used to iden...
Assignment of the gene for human intra-acrosomal protein SP-10 to the p12----q13 region of chromosome 11
Spermatogenesis testis-specific gene somatic
2009/2/7
The human sperm antigen SP-10 is a testis-specific, intra-acrosomal protein associated with the membranes of the acrosomal vesicle. The molecule has been designated a "primary vaccine candidate" b...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Effect of sperm-associated antibodies on the acrosomal status of human sperm
Antisperm antibodies acrosome reaction infertility spermatozoa
2009/2/6
The acrosomal status of human sperm was assessed by the specific binding of Pisum sativum lectin to the acrosomal matrix. Immunoglobulin G (IgG) fractions of plasmas that were positive for IgG antis...
Partial characterization of an intra-acrosomal protein, human acrin1（MN7）
intra-acrosomal protein human acrin1（MN7）
2008/12/26
Acrin1 (MN7), a 90-kd glycoprotein, is localized in the sperm acrosome of several rodents. The purpose of this study was to determine the molecular size and subcellular localization of acrin1 (MN7) in...
The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation
reactive oxygen species equine sperm motility viability
2008/12/25
The objective of this study was to examine the influence of reactive oxygen species (ROS), generated through the use of the xanthine (X)-xanthine oxidase (XO) system, on equine sperm motility, via...
Calpain and calpastatin are located between the plasma membrane and outer acrosomal membrane of cynomolgus macaque spermatozoa
Calpain calpastatin plasma membrane outer acrosomal membrane cynomolgus macaque spermatozoa
2008/12/25
Mammalian sperm must undergo an acrosome reaction prior to penetration of the zona pellucida and subsequent fusion with an oocyte. Sperm gain the capability to acrosome react after a period of capac...