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南京农业大学农学院《Molecular Plant》发表万建民院士团队“The RNA binding protein EHD6 recruits the m6A reader YTH07 and sequesters OsCOL4mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering”（图）
万建民 蛋白 相分离过程
2024/6/11
2024年5月9日，万建民院士团队与北京大学贾桂芳团队合作在Molecular Plant发表了题为The RNA binding protein EHD6 recruits the m6A reader YTH07 and sequesters OsCOL4mRNA into phase-separated ribonucleoprotein condensates to promote ric...
【北京大学物理化学讲座】The route going from transition path time distributions and protein folding times, to quantum mechanics, tunneling times and nonadiabatic transition times（图）
route going transition path time distributions quantum mechanics
2023/6/19
【北京大学物理化学讲座】The route going from transition path time distributions and protein folding times, to quantum mechanics, tunneling times and nonadiabatic transition times
Measurement of Solvation Responses at Multiple Sites in a Globular Protein
background electrolyte capillary electrophoresis humic acid
2016/5/24
Proteins respond to electrostatic perturbations through complex reorganizations of their charged and polar groups, as well as those of the surrounding media. These solvation responses occur both in th...
Antibody Evolution Constrains Conformational Heterogeneity by Tailoring Protein Dynamics
flexibility nonlinear spectroscopy fluorscein molecular recognition
2016/5/24
The evolution of proteins with novel function is thought to start from precursor proteins that are conformationally heterogeneous. The corresponding genes may be duplicated and then mutated to select ...
Protonation,Photobleaching,and Photoactivation of Yellow Fluorescent Protein (YFP 10C):A Unifying Mechanism
Protonation Photobleaching Photoactivation Yellow Fluorescent Protein Unifying Mechanism
2016/5/24
Yellow fluorescent protein (YFP 10C) is widely used as a probe in biology, but its complex photochemistry gives rise to unusual behavior that requires fuller definition. Here we characterize the kinet...
Vibrational Stark Spectroscopy of NO Bound to Heme:Effects of Protein Electrostatic Fields on the NO Stretch Frequency
rare earth alloys X-ray diffraction crystal structure phase diagram
2016/5/23
The vibrational Stark effect measures the effect of an external electric field on the vibrational (IR) spectrum of a molecule. This technique gives quantitative information on the sensitivity of a vib...
The Role of the Distal and Proximal Protein Environments in Controlling the Ferric Spin State and in Stabilizing Thiolate Ligation in Heme Systems:Thiolate Adducts of the Myoglobin H93G Cavity Mutant
salicylic alcohols hydrogen bonds quantum chemistry
2016/5/23
Recently, heme protein cavity mutants have been engineered in which the proximal coordinating amino acid has been replaced by a smaller, noncoordinating residue leaving a cavity that can be filled by ...
Protein Relaxation Dynamics in Human Myoglobin
Protein Relaxation Dynamics Human Myoglobin
2016/5/23
Transient absorption spectra in the Soret region have been measured following the photolysis of human MbCO in 75%(w/w) glycerol:water at 250, 270, and 290 K. The peak of the transient difference spect...
Large Protein-Induced Dipoles for a Symmetric Carotenoid in a Photosynthetic Antenna Complex
Bacterial Proteins Carotenoids Cell Membrane Chemistry, Physical Macromolecular Substances Membrane Potentials Membrane Proteins Photosynthesis Physicochemical Phenomena Rhodobacter sphaeroides Solvents Spectrum Analysis
2016/5/23
Unusually large electric field effects have been measured for the absorption spectra of carotenoids (spheroidene) in the B800-850 light-harvesting complex from the photosynthetic bacterium Rhodobacter...
Nonphotochemical Holeburning in a Protein Matrix: Chlorophyllide in Apomyoglobin
Proteins Polymers Temperature measurement Amorphous metals Dephasing
2016/5/20
Nonphotochemical holeburning spectroscopy has been used to study a chlorophyllide chromophore substituted for heme in apomyoglobin. The observed holewidths were compared with those found for the same ...
Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.
Chemical cross-linking/mass spectrometry targeting acidic residues proteins protein complexes.
2015/12/11
The study of proteins and protein complexes using chemical cross-linking followed by the MS identification of the cross-linked peptides has found increasingly widespread use in recent years. Thus far,...
Development of Mass Spectrometry-Based Methods for Quantitation and Characterization of Protein Drugs: Transferrin as a Model Drug Delivery Vehicle
Mass Spectrometry O18 labeling Protein quantitation Transferrin
2014/11/20
In the last two decades, protein drugs have enjoyed a rapid growth and achieved a tremendous success in treating human diseases. However, the presence of physiological barriers greatly impedes the eff...
Modeling Spatial Correlation of DNA Deformation: DNA Allostery in Protein Binding
Modeling Spatial Correlation DNA Deformation DNA Allostery Protein Binding
2014/9/24
We report a study of DNA deformations using a coarsegrained mechanical model and quantitatively interpret the allosteric effects in protein−DNA binding affinity. A recent single-molecule study (...
Characterisation of polystyrene coatings after plasma immersion ion implantation and adsorption of protein
Plasma immersion ion implantation X-ray reflectometry neutron reflectometry ellipsometry protein polystyrene
2012/4/20
A polystyrene film spun onto polished silicon substrates was implanted with either nitrogen or argon ions using plasma immersion ion implantation (PIII) and subsequently investigated by X-ray and neut...
Entropy-Enthalpy Compensation May Be a Useful Interpretation Tool for Complex Systems Like Protein-DNA Complexes: An Appeal to Experimentalists
Entropy-Enthalpy Compensation Useful Interpretation Tool Complex Systems Like Protein-DNA Complexes Experimentalists
2012/2/29
In various chemical systems enthalpy-entropy compensation (EEC) is a well-known rule of behavior, although the physical roots of it are still not completely understood. It has been frequently question...