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Characteristics and Gel Properties of Gelatin from Goat Skin as Influenced by Alkaline-pretreatment Conditions
Goat Gelatin Skin Pretreatment Alkaline Gel Strength
2016/7/27
Characteristics and properties of gelatin from goat skin pretreated with NaOH solutions (0.50 and 0.75 M) for various times (1 to 4 days) were investigated. All gelatins contained α-chains as the pred...
利用超载洗脱技术在TSK Gel CM-5PW分析柱上进行溶菌酶百毫克级制备
分离 溶菌酶 纯化 高效毛细管电泳
2007/12/5
本实验采用非线性色谱的展开方式之一-超载洗脱, 在普通的分析型离子交换柱TSK Gel-5PW (Φ7.5×75mm)上, 一次进样蛋白质混合样150mg, 成功地进行了溶菌酶的分离纯化, 回收率达90%。收集的馏份经透析和冰冻干燥后,通过高效毛细管电泳(HPCE)测定纯度, 得到了满意的结果, 活性回收率达88%。
Inconsistency in the Improvements of Gel Strength in Chicken and Pork Sausages Induced by Microbial Transglutaminase
Texture Chicken Pork Microbial Transglutaminase Gel Improvement Protein Crosslinking
2016/4/26
This research investigated variation in the improvement of the texture of chicken and pork sausages induced by microbial transglutaminase (MTG). The extractability of myofibrillar proteins from these ...
Gel Color and Texture of Surimi-like Pork from Muscles at Different Rigor States Post-mortem
Surimi-like Pork Gel Color Texture Muscle Rigor States
2016/4/26
Gels were made from surimi-like pork (SLP) made from muscles obtained at 1, 24 and 72 h post-mortem. The SLP from pre-rigor muscle had higher pH and moisture percentage compared to in- or post-rigor m...
Proteomic Comparison between Japanese Black and Holstein Cattle by Two-dimensional Gel Electrophoresis and Identification of Proteins
Cattle Meat Quality Two-dimensional Gel Electrophoresis LC-MS/MS Proteome
2016/4/25
Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was t...
Effect of Disodium Fumarate on In vitro Rumen Fermentation of Different Substrates and Rumen Bacterial Communities as Revealed by Denaturing Gradient Gel Electrophoresis Analysis of 16S Ribosomal DNA
Disodium Fumarate In vitro Fermentation Denaturing Gradient Gel Electrophoresis
2016/4/25
Two experiments were conducted to investigate the effects of disodium fumarate on the in vitro rumen fermentation profiles of different substrates and microbial communities. In experiment 1, nine diet...
Application of Gel-based Proteome Analysis Techniques to Studying Post-mortem Proteolysis in Meat
Proteolysis Proteome Analysis Skeletal Muscle
2016/4/18
This study was conducted to evaluate the possible application of 2 D-SDS-PAGE (2 DE)-based proteome analysis techniques to the assessment of extreme proteolysis in postmortem skeletal muscle. Eight Ha...
Microbial Transglutaminase Modifies Gel Properties of Porcine Collagen
Porcine Collagen Gel Microbial Transglutaminase Structural and Physicochemical Properties Melting Point
2016/4/12
We studied the gel properties of porcine collagen with microbial transglutaminase (MTGase) as a catalyst. A creep meter was used to measure the mechanical properties of gel. The results showed samples...