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Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Porcine Oocyte Antioxidants Quercetin Reactive Oxygen Species [ROS]
2016/5/19
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate ...
Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
Brilliant Cresyl Blue Embryonic Development Subcellular Characteristics
2016/5/19
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogena...
Rapamycin Rescues the Poor Developmental Capacity of Aged Porcine Oocytes
Reactive Oxygen Species (ROS) Mammalian Target of Rapamycin (mTOR)
2016/5/16
Unfertilized oocytes age inevitably after ovulation, which limits their fertilizable life span and embryonic development. Rapamycin affects mammalian target of rapamycin (mTOR) expression and cytoskel...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes
Cryoprotectant Porcine Oocytes ATP Content
2016/4/29
This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs)...
Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts
Parthenogenetic Activation Oocyte Embryonic Stem Cells Porcine
2016/4/26
These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-...
MAP Kinase is Activated dring the Maturation of Porcine Oocytes
Porcine Oocytes Maturation Western Blotting MAP Kinase Phosphorylation
2016/4/15
In an attempt to evaluate the function of MAP kinase in porcine oocytes and to develop a method of the assessment of its activity, myelin basic protein (MBP) was used as a substrate to detect the MAP ...
Effects of Meiotic Stages, Cryoprotectants, Cooling and Vitrification on the Cryopreservation of Porcine Oocytes
Cryopreservation Cryoprotectants Porcine Oocytes Cooling Vitrification
2016/4/8
Different factors may affect the sensitivity of porcine oocytes during cryopreservation. The effect of two methods (cooling and vitrification), four cryoprotectants [glycerol (GLY), 1, 2-propanediol (...